Cytoprotective Effects of Lactobacilli on Mouse Epithelial Cells during Salmonella Infection
نویسندگان
چکیده
Treatment of common pathogens, such as Salmonella species, Escherichia coli, Staphylococcus aureus, etc., is a big challenge for practitioner. Antibiotics’ side effects during their application the treatment infectious diseases should not be underestimated they have many issues, transfer antibiotics-resistant genes, dysbiosis, and antibiotic-resistant strains, which main hurdle in eradication diseases. To avoid these antibiotics complications, modern countries, interest using probiotics feed supplementation to promote health prevent or treat intestinal has been increasing. The purpose present study was evaluate probiotic potential three Lactobacilli strains isolated from clinically healthy dogs further utilization dietary supplement pathogenic antibiotic complication. After 16SrRNA sequencing, vitro tests were conducted assess survival under simulated gastrointestinal conditions adhesion ability MODE-K cell line, on epithelial barrier function, anti-inflammatory activities, host defensin peptides (beta-defensin 3), inhibitory pathogens. showed considerable survive environmental conditions, low pH, high bile salt concentrations along with good properties cells. Pathogenic bacterial growth cells significantly inhibited by Lactobacilli. Real-time PCR analyses demonstrated that L. acidophilus strain AR1 AR3 inhibit Salmonella-induced proinflammatory cytokine (IL-6, IL-8, IL-1β) production reinforce expression tight junction protein (occludin). None induce mRNA beta-defensin 3 Based results, supplemented canine feed. However, vivo studies investigating health-promoting are awaited.
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ژورنال
عنوان ژورنال: Fermentation
سال: 2022
ISSN: ['2311-5637']
DOI: https://doi.org/10.3390/fermentation8030101